Anti-Cytokeratin 8 Purified

• Catalog Number: orb43712
• Category: Antibodies
• Description: Mouse Monoclonal to Cytokeratin 8.
• Clonality: Monoclonal
• Clone Number: C-43
• Tested applications: FC, ICC, IHC-P, IP, WB
• Reactivity: Bovine, Human, Porcine, Rabbit, Sheep
• Isotype: Mouse IgG1
• Immunogen: Cytoskeleton preparation from HeLa human cervix carcinoma cell line.
• Concentration: 1 mg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: Cytokeratin 8
• Entrez: 3856
• UniProt ID: Q7L4M3
• RRID: AB_10991021
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-Cytokeratin 8 antibody
• Note: For research use only
• Application notes: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: prostate. Flow cytometry: Recommended dilution: 0.5-4 μg/ml. Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Western blotting detection of cytokeratin 8 in HeLa cell lysate (reducing conditions) by mouse monoclonal antibody C-43.

Immunocytochemistry staining of cytokeratin 8 in Hep-2 cells using mouse monoclonal C-43 (orb43712, diluted 1:100), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green).

Immunohistochemistry staining of human prostate (paraffin sections) using anti-cytokeratin 8(C-43).