Cytokeratin 18 Antibody

SKU: orb43750

Description

Mouse monoclonal antibody to Cytokeratin 18

Images & Validation

• Tested Applications: ELISA, FC, ICC, IHC-P, IP, WB
• Reactivity: Human
• Application Notes:
  Western blotting: Recommended dilution: 1-2 μg/ml. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: colon, skin. Immunocytochemistry: positive control: MCF-7 human breast adenocarcinoma cell line, HeLa human cervix carcinoma cell line. ELISA: Positive control: MCF-7 human breast adenocarcinoma cell line; the antibody DC-10 has been tested as the detection antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with the antibody C-04 (cat. no. orb43702). Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: DC-10
• Immunogen: Human breast carcinoma cell line PMC-42.
• Target: Cytokeratin 18
• Purification: Purified by protein-A affinity chromatography.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

K18, CK18, CYK18, KRT18

Separation of MCF-7 cells (red-filled) from Caco-2 cells (black-dashed) in flow cytometry analysis (intracellular staining) of cells stained using anti-cytokeratin 18 (DC-10) purified antibody (concentration in sample 0.6 μg/ml, GAM APC).

Immunocytochemistry staining of cytokeratin 18 in Hep-2 cells using mouse monoclonal antibody DC-10 (orb43750, diluted 1:400), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green), cell nuclei stained with PI (1 µg/ml; orange).

Immunohistochemistry staining of human colon (paraffin sections) using anti-cytokeratin 18 (clone DC-10).

Western blotting analysis of human cytokeratin 18 using mouse monoclonal antibody DC-10 on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-cytokeratin 18 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for cytokeratin 18 at approximately 46 kDa, and its proteolytic fragment at approximately 25 kDa.

Quick Database Links

Gene Symbol

Cytokeratin 18

UniProt

P05783

NCBI Gene ID

3875