Anti-Cytokeratin 18 Purified

• Catalog Number: orb43702
• Category: Antibodies
• Description: Mouse monoclonal antibody to Cytokeratin 18
• Clonality: Monoclonal
• Clone Number: C-04
• Tested applications: ELISA, FC, ICC, IHC-P, IP, WB
• Reactivity: Mammal
• Isotype: Mouse IgG1
• Immunogen: Cytoskeleton preparation of epidermal carcinoma cell line A431.
• Concentration: 1 mg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: Cytokeratin 18
• Entrez: 3875
• UniProt ID: P05783
• RRID: AB_10990215
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti-CYK 18 antibody, anti-CYK18 antibody, anti-CK
• Note: For research use only
• Application notes: Western blotting: Recommended dilution: 1-2 μg/ml.Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive control: colon, kidney. ELISA: The antibody C-04 has been tested as the capture antibody in a sandwich ELISA for analysis of cytokeratin 18 in combination with the antibody DA-7 (cat. no. 1B-110-C100) and with the antibody DC-10 (cat. no. 1B-107-C100). Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Separation of HeLa cells stained using anti-cytokeratin 18 (C-04) purified antibody (concentration in sample 0.6 μg/ml, GAM FITC, red-filled) from HeLa cells unstained by primary antibody (GAM FITC, black-dashed) in flow cytometry analysis (intracellular staining) of HeLa cell suspension.

Flow cytometry intracellular staining pattern of HeLa cell suspension stained using anti-cytokeratin 18 (C-04) purified antibody (concentration in sample 0.6 μg/ml) GAM FITC.

Immunohistochemistry staining of human kidney (paraffin-embedded sections) with anti-Cytokeratin 18 (C-04).

Immunohistochemistry staining of human colon (paraffin-embedded sections) with anti-Cytokeratin 18 (C-04).

Western blotting analysis of human cytokeratin 18 using mouse monoclonal antibody C-04 on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-cytokeratin 18 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for cytokeratin 19 at approximately 46 kDa.