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Anti-Cytokeratin 18 Biotin
Catalog Number: orb43751
| Catalog Number | orb43751 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody against Cytokeratin 18 conjugated to Biotin |
| Clonality | Monoclonal |
| Clone Number | DC-10 |
| Tested applications | ELISA, FC, ICC, IHC-P, IP, WB |
| Reactivity | Human |
| Isotype | Mouse IgG1 |
| Immunogen | Human breast carcinoma cell line PMC-42. |
| Concentration | 1 mg/ml |
| Purity | Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography. |
| Conjugation | Biotin |
| Target | Cytokeratin 18 |
| Entrez | 3875 |
| UniProt ID | P05783 |
| RRID | AB_10990062 |
| Storage | Store at 2-8°C. Do not freeze. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | anti-CYK 18 antibody, anti-CYK18 antibody, anti-CK Read more... |
| Note | For research use only |
| Application notes | Flow cytometry: Recommended dilution: 2-6 μg/ml. Intracellular staining.Western blotting: Recommended dilution: 1-2 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
Flow cytometry analysis (intracellular staining) of HeLa cells with anti-human cytokeratin 18 (DC-10) biotin antibody (red, concentration in sample 6 μg/ml) streptavidin-APC, with blank sample (black).
Western blotting analysis of human cytokeratin 18 using mouse monoclonal antibody DC-10 on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of biotinylated mouse anti-cytokeratin 18 monoclonal antibody followed by IRDye800-conjugated streptavidin. Cytokeratin 18 was detected at approximately 46 kDa.
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