Anti-CRP [P24]

• Catalog Number: orb1463341
• Category: Antibodies
• Description: Mouse monoclonal antibody to CRP
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: P24
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: The original antibody was generated by immunizing BALB/c mice with C-reactive protein extracted from human plasma.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CRP
• UniProt ID: P02741
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: C reactive protein; PTX1; C-reactive protein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of HepG2 cells with anti-CRP P24. Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells stained with the chimeric rabbit IgG version of P24 (orb1463341) (1:1000 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1463341, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody (orb256390) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CRP antibody P24. Human liver(A) (0.0003 µg/ml), kidney(B) (0.0003 µg/ml), staining in human plasma(C) (0.001 µg/ml) and staining in human serum(D) (0.01 µg/ml) tissue lysate (35 µg protein in RIPA buffer) wer resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of P24 (orb1463341) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.