Anti-CRISPR-Cas9 SP Rabbit Monoclonal Antibody

• Catalog Number: orb548511
• Category: Antibodies
• Description: Anti-CRISPR-Cas9 SP Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Recombinant fragment, Streptococcus pyogenes.
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: ABCI-3
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Bacteria
• Isotype: Rabbit IgG
• Immunogen: Recombinant fragment derived from Streptococcus pyogenes.
• Antibody Type: Primary Antibody
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 50 kDa
• UniProt ID: Q99ZW2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CRISPR-associated endonuclease Cas9/Csn1; 3.1.-.-;
• Note: For research use only
• Application notes: WB 1:1000-1:5000IHC 1:50-1:200ICC/IF 1:50-1:200FC 1:50. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CRISPR-Cas9 SP expression in (1) 293T cell lysate transfected with CRISPR-Cas9; (2) 293T cell lysate; (3) 3T3 cell lysate; (4) PC12 cell lysate.

Immunofluorescent analysis of 293T cells transfected with CRISPR-SpCas9, using CRISPR-Cas9 SP Antibody.