Anti-CREB (pS121) Antibody

• Catalog Number: orb1423567
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CREB (pS121).
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: CREB1
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Porcine, Rat, Virus, Zebrafish
• Dilution range: WB: WB (1/500 - 1/1000), IH (1/100 - 1/200), E: WB (1/500 - 1/1000), IH (1/100 - 1/200)
• Conjugation: Unconjugated
• UniProt ID: P16220
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Cyclic AMP-responsive element-binding protein 1; C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Direct ELISA antibody dose-response curve using Anti-CREB (pS121) Antibody. Antigen (phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.

Immunohistochemical analysis of CREB (pS121) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of CREB (pS121) expression in DLD (A) whole cell lysates.