Anti-COX4-1 Antibody

• Catalog Number: orb1474397
• Category: Antibodies
• Description: Mouse monoclonal antibody to COX4-1
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: FC, IF, IH, IP, WB
• Reactivity: Goat, Hamster, Human, Mouse, Primate, Rat
• Immunogen: A synthetic peptide corresponding to carboxyl terminal residues of human COX IV
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100), IP (1/10 - 1/50), FC (1/50 - 1/100)
• Form/Appearance: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Conjugation: Unconjugated
• Target: COX4I1
• Entrez: 1327, 12857, 29445
• UniProt ID: P10888, P19783, P13073
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Alternative names: COX4; Cytochrome c oxidase subunit 4 isoform 1 mit
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of COX4-1 expression in CHOk1 (A), COS7 (B), NIH3T3 (C), Hela (D), C6 (E), mouse skeletal muscel (F) whole cell lysates. (Predicted band size: 20 kD; Observed band size: 17 kD)

Immunohistochemical analysis of COX4-1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.50). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of COX4-1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Flow cytometric analysis of COX4-1 in K562 (red). Blue line histogram represents the isotype control.