Anti-COVID-19 & SARS-CoV S glycoprotein [CR3022]

• Catalog Number: orb669766
• Category: Antibodies
• Description: Mouse monoclonal antibody to COVID-19 & SARS-CoV S glycoprotein
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: CR3022
• Tested applications: ELISA, IF
• Reactivity: Virus
• Isotype: IgG1
• Immunogen: The original monoclonal antibody was generated through an scFv library derived from a peripheral blood lymphocytes of a patient exposed to the SARS-CoV.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: COVID-19 & SARS-CoV S glycoprotein
• UniProt ID: P59594
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Spike protein, COVID19, COVID 19, S protein, SARS-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Binding curve of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 (orb669766) to SARS-CoV-2 Spike Glycoprotein (S1), Sheep Fc-Tag and SARS-CoV-2 Spike Glycoprotein (S2), Sheep Fc-Tag from HEK293 cells. ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), Sheep Fc-Tag (blue line) or SARS-CoV-2 Spike Glycoprotein (S2), Sheep Fc-Tag (orange line) from HEK293 cells (The Native Antigen Company) at concentrations of 5 µg/ml. A 3-fold serial dilution from 125 ng/ml was performed using orb669766. For detection, a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.

Binding curve of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 (orb669766) to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 of various origin. ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), His-Tag (Insect Cells; grey line), SARS-CoV-2 Spike Glycoprotein (S2), His-Tag (Insect Cells; yellow line) and SARS Coronavirus Spike Glycoprotein (S1), His-Tag (HEK293 cells; blue line) (The Native Antigen Company) at concentrations of 5 µg/ml. A 3-fold serial dilution from 41.6 ng/ml was performed using orb669766. For detection, a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.

Binding curve of four different formats of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 (His-Tag (Insect Cells)). ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), His-Tag (Insect Cells; grey line) and SARS-CoV-2 Spike Glycoprotein (S2), His-Tag (Insect Cells; yellow line) (Native Antigen) at concentrations of 5 µg/ml.

ELISA using CR3022 and the Brazilian mutant Spike protein (B1.1.24 / P.1). The plate was coated with the mutant Spike protein P.1 (The Native Antigen Company) at 2.5 µg/ml.