Anti-Collagen 5 alpha 1 Antibody

• Catalog Number: orb213762
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Collagen V
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Collagen 5 alpha 1. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500, IP: 1-10-1-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: COL5A1
• Entrez: 85490, 12831, 1289
• UniProt ID: O88207, Q9JI03, P20908
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti Alpha 1 type V collagen antibody, anti Alpha
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Zhicheng Sun 1, Chengxiong Huang 2, Zheming Cao 2, Yu Xiao 2, Panfeng Wu 2, Xiaoyang Pang 1, Yan Yang PF127/bleomycin hydrogel promotes subcutaneous extracellular matrix remodeling and fibrosis to construct personalized flaps through the TGFβ-Col signaling pathway Immunopharmacol Immunotoxicol, (2024)

PubMed: 39164805

Applications

WB

Reactivity

Mouse

Western blot analysis of Collagen 5 alpha 1 expression in mouse testis (A), C6 (B), CT26 (C), U87MG (D) whole cell lysates. (Predicted band size: 183 kD; Observed band size: 220 kD)

Immunohistochemical analysis of Collagen 5 alpha 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Collagen 5 alpha 1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).