Anti-Collagen 2 alpha 1 Antibody

• Catalog Number: orb235107
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Collagen II alpha 1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Collagen 2 alpha 1. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: COL2A1
• Entrez: 12824, 1280, 25412
• UniProt ID: P02458, P05539, P28481
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Collagen alpha-1(II) chain; Alpha-1 type II collag
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Tao, Haitao et al. Downregulation of miR-34a Promotes Proliferation and Inhibits Apoptosis of Rat Osteoarthritic Cartilage Cells by Activating PI3K/Akt Pathway Clin Interv Aging, 15, 373-385 (2020)

PubMed: 32214804

Western blot analysis of Collagen 2 alpha 1 expression in Panc1 (A) whole cell lysates. (Predicted band size: 141 kD; Observed band size: 220 kD)

Immunohistochemical analysis of Collagen 2 alpha 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.