Anti-Collagen 1 alpha 2 Antibody

• Catalog Number: orb213757
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Collagen I
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, WB
• Reactivity: Human, Mouse, Porcine, Rat, Sheep
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Collagen 1 alpha 2. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: COL1A2
• Entrez: 84352, 12843, 1278
• UniProt ID: P08123, P02466, Q01149
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-CO1A1_HUMAN antibody, anti-Collagen I antibod
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Ryu, Jina et al. Evaluation of Three-Dimensional Bioprinted Human Cartilage Powder Combined with Micronized Subcutaneous Adipose Tissues for the Repair of Osteochondral Defects in Beagle Dogs Int J Mol Sci, 23, 2743 (2022)

PubMed: 35269885

Western blot analysis of Collagen 1 alpha 2 expression in rat ovary (A), Hela (B) whole cell lysates. (Predicted band size: 129 kD; Observed band size: 130 kD)

Immunohistochemical analysis of Collagen 1 alpha 2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Collagen 1 alpha 2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).