Anti-CNOT4 Antibody

• Catalog Number: orb1880392
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CNOT4
• Clonality: Polyclonal
• Tested applications: FC, IH, WB
• Reactivity: Human, Mouse
• Immunogen: Recombinant fusion protein of human CNOT4. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100), FC (1/10 - 1/30)
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Entrez: 53621, 4850
• UniProt ID: O95628, Q8BT14
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Alternative names: NOT4; CCR4-NOT transcription complex subunit 4; CC
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CNOT4 expression in mouse cerebellum (A) whole cell lysates. (Predicted band size: 63 kD; Observed band size: 63 kD)

Immunohistochemical analysis of CNOT4 staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Flow cytometric analysis of U2OS cells using Anti-CNOT4 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.