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Anti-Claudin 1/CLDN1 Antibody

Catalog Number: orb669070

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb669070
CategoryAntibodies
DescriptionAnti-Claudin 1/CLDN1 Antibody. Tested in IF, IHC, ICC applications. This antibody reacts with Human.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsICC, IF, IHC
ReactivityHuman
IsotypeRabbit IgG
ImmunogenA synthetic peptide corresponding to a sequence in the middle region of human Claudin 1/CLDN1, identical to the related mouse and rat sequences.
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW34 kDa
UniProt IDO95832
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesGrowth/differentiation factor 15; GDF-15; Macropha
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NoteFor research use only
Application notesImmunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human Immunocytochemistry/Immunofluorescence, 5μg/ml, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
Expiration Date12 months from date of receipt.
Anti-Claudin 1/CLDN1 Antibody

IF analysis of Claudin 1/CLDN1 using anti-Claudin 1/CLDN1 antibody. Claudin 1/CLDN1 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL rabbit anti-Claudin 1/CLDN1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-Claudin 1/CLDN1 Antibody

IF analysis of Claudin 1/CLDN1 using anti-Claudin 1/CLDN1 antibody. Claudin 1/CLDN1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL rabbit anti-Claudin 1/CLDN1 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-Claudin 1/CLDN1 Antibody

IHC analysis of Claudin 1/CLDN1 using anti Claudin 1/CLDN1 antibody. Claudin 1/CLDN1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Claudin 1/CLDN1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-Claudin 1/CLDN1 Antibody

IHC analysis of Claudin 1/CLDN1 using anti Claudin 1/CLDN1 antibody. Claudin 1/CLDN1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Claudin 1/CLDN1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-Claudin 1/CLDN1 Antibody

IHC analysis of Claudin 1/CLDN1 using anti Claudin 1/CLDN1 antibody. Claudin 1/CLDN1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Claudin 1/CLDN1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

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    IHC-P,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    80 μl
  • Claudin 1 (CLDN1) Antibody (Loop2) [orb36512]

    WB

    Rat

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    80 μl
  • Anti-Claudin 1/CLDN1 Antibody [orb1474863]

    ELISA,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
  • Anti-Claudin 1 CLDN1 Rabbit Monoclonal Antibody [orb547796]

    WB

    Human, Mouse, Rat

    Rabbit

    Monoclonal

    Unconjugated

    30 μl, 100 μl
  • Anti-Claudin 1/CLDN1 Antibody [orb2591119]

    ELISA,  WB

    Human

    Rabbit

    Polyclonal

    iFluor647

    100 μg