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Anti-Claudin 1/CLDN1 Antibody

Catalog Number: orb1474863

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb1474863
CategoryAntibodies
DescriptionAnti-Claudin 1/CLDN1 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, WB
ReactivityHuman
IsotypeRabbit IgG
ImmunogenE.coli-derived human Claudin 1/CLDN1 recombinant protein (Position: F96-V211).
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW22 kDa
UniProt IDO95832
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesGrowth/differentiation factor 15; GDF-15; Macropha
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NoteFor research use only
Application notesWestern blot, 0.1-0.25 μg/ml, Human ELISA, 0.1-0.5 μg/ml, -. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
Expiration Date12 months from date of receipt.
Anti-Claudin 1/CLDN1 Antibody

Western blot analysis of Claudin 1/CLDN1 using anti-Claudin 1/CLDN1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Claudin 1/CLDN1 antigen affinity purified polyclonal antibody at 0.25 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Claudin 1/CLDN1 at approximately 22 kDa. The expected band size for Claudin 1/CLDN1 is at 28 kDa.

  • Anti-Claudin 1/CLDN1 Antibody [orb669070]

    ICC,  IF,  IHC

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μg, 10 μg
  • Claudin 1 (CLDN1) Antibody (Loop1) [orb36511]

    IHC-P,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    80 μl
  • Claudin 1 (CLDN1) Antibody (Loop2) [orb36512]

    WB

    Rat

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    80 μl
  • Anti-Claudin 1 CLDN1 Rabbit Monoclonal Antibody [orb547796]

    WB

    Human, Mouse, Rat

    Rabbit

    Monoclonal

    Unconjugated

    30 μl, 100 μl
  • Anti-Claudin 1/CLDN1 Antibody [orb2591119]

    ELISA,  WB

    Human

    Rabbit

    Polyclonal

    iFluor647

    100 μg