Anti-CD74(milatuzumab biosimilar) mAb

• Catalog Number: orb1826918
• Category: Antibodies
• Description: Anti-CD74(milatuzumab biosimilar) mAb
• Species/Host: Human Humanized
• Clonality: Monoclonal
• Tested applications: ELISA, FC
• Reactivity: Human
• Isotype: IgG1(K97R)
• Dilution range: ELISA 1:5000-10000, Flow Cyt 1:100
• Conjugation: Unconjugated
• Target: CD74
• UniProt ID: P04233
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: DHLAG
• Note: For research use only
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis with 15 μg/mL Anti-CD74 (milatuzumab biosimilar) mAb (orb1826918) on Expi293 cells transfected with Human CD74 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human CD74 Protein, hFc Tag (orb1173822) can bind Anti-CD74 (milatuzumab biosimilar) mAb (orb1826918) in a linear range of 0.64–16 ng/ml. In order to specifically detect orb1826918, mouse anti-human Fab-specific antibody was used as detection antibody.

Flow cytometry analysis of antigen binding of anti-human CD74 mAb (orb1826918). (A) orb1826918 does not bind to 293T cells that do not express CD74. (B) A clear peak shift of orb1826918 was seen compared to the control when incubated with CD74-expressing Raji cells, indicating strong binding of orb1826918 to CD74. Antibodies were incubated at 5 μg/mL.