Anti-CD47 [mIAP430]

• Catalog Number: orb1152502
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD47
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: mIAP430
• Tested applications: Blocking, IF
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: This antibody was raised by immunising IAP-deficient mice with purified human placental CD47.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD47
• UniProt ID: Q08722
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: IAP; C5/D5; Leukocyte surface antigen CD47; Antige
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the anti-CD47 antibody mIAP430. Jurkat cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of mIAP430 (orb1152502, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of Jurkat cells using anti-CD47 antibody mIAP430. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of mIAP430 (orb1152502) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1152502, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (orb256390) followed by Alexa Fluor® 488 secondary antibody.