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Catalog Number | orb1090001 |
---|---|
Category | Antibodies |
Description | Rabbit monoclonal antibody to CD44 (Indian blood group) |
Species/Host | Rabbit |
Clonality | Monoclonal |
Clone Number | SAIC-01B-4D8 |
Tested applications | ELISA, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Peptide "TFIPVTSAK" derived from CD44, a hyaluronic acid receptor mediating cell-cell and cell-matrix interactions , conjugated to KLH. |
Concentration | 1 mg/ml |
Purity | Purified |
Conjugation | Unconjugated |
Target | CD44 (Indian blood group) |
UniProt ID | P16070 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | LHR; MDU2; MDU3; MIC4; Extracellular matrix recept Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow cytometry using the Anti-CD44 (Indian blood group) antibody SAIC-01B-4D8. Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of SAIC-01B-4D8 (orb1090001, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Immunofluorescence staining of HeLa cells with anti-CD44 (Indian blood group) SAIC-01B-4D8. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells stained with the chimeric rabbit IgG version of SAIC-01B-4D8 (orb1090001) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1090001, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.