Anti-CD4 (pS433) Antibody

• Catalog Number: orb1421642
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CD4 (pS433).
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: CD4
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Dilution range: WB: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200), IF: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200), E: WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
• Conjugation: Unconjugated
• UniProt ID: P01730
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: T-cell surface glycoprotein CD4; T-cell surface an
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CD4 (pS433) expression in mouse spleen (A), rat spleen (B) whole cell lysates.

Direct ELISA antibody dose-response curve using Anti-CD4 (pS433) Antibody. Antigen (phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.

Immunohistochemical analysis of CD4 (pS433) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of CD4 (pS433) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.