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Item 1 of 3
Anti-CD4 (Phospho-S433) Antibody
Catalog Number: orb1289874
| Catalog Number | orb1289874 |
|---|---|
| Category | Antibodies |
| Description | Rabbit Polyclonal antibody to CD4 |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, WB |
| Reactivity | Human, Mouse, Primate, Rat |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S433 of human CD4 protein. The exact sequence is proprietary. |
| Dilution range | WB: 1:500-1:1000, IH: 1:50-1:200, IF:IC: 1:50-1:200 |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Conjugation | Unconjugated |
| Target | CD4 |
| Entrez | 920, 24932, 12504 |
| UniProt ID | P01730, P05540, P06332 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Alternative names | T-cell surface glycoprotein CD4; T-cell surface an Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of CD4 (Phospho-S433) expression in mouse spleen (A), rat spleen (B) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 56 kD)
Immunofluorescent analysis of CD4 (Phospho-S433) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Direct ELISA antibody dose-response curve using Anti-CD4 (Phospho-S433) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
