Anti-CD4 [MT310]

• Catalog Number: orb348853
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD4
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MT310
• Tested applications: Blocking, FC, IF, IHC-P, IP, NeA
• Reactivity: Human, Primate
• Isotype: IgG1
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD4
• UniProt ID: P01730
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Leu3/T3; T-cell differentiation antigen; Ly-4; L3T
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Herzog, C. et al. Monoclonal anti-CD4 in arthritis Lancet, 2, 1461-1462 (1987)

PubMed: 2892019

Flow-cytometry using anti-CD4 antibody MT310 Cynomolgus monkey lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of MT310 (orb348912, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Flow-cytometry using anti-CD4 antibody MT310 Human lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of MT310 (orb348912, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Flow-cytometry using anti-CD4 antibody MT310 Rhesus monkey lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of MT310 (orb348912, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Immunohistochemical staining of guinea pig skin tissue using anti-CD4 antibody MT310 Anti-CD4 staining of formaldehyde fixed paraffin embedded guinea pig skin tissue, at 40x magnification. The mouse IgG1-chimeric version of MT310 (orb348853) was used to stain samples at a concentration of 3 µg/ml.