Anti-CD4 [CE9.1 (Clenoliximab)]

• Catalog Number: orb348883
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD4
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: CE9.1 (Clenoliximab)
• Tested applications: Blocking, ELISA, FC, IHC
• Reactivity: Human, Monkey
• Isotype: Human IgG
• Immunogen: Domain 1 of human CD4.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD4
• UniProt ID: P01730
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: p55; T cell surface antign T4/Leu-3.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the anti-CD4 research biosimilar antibody Clenoliximab (CE9.1) Human lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of Clenoliximab (orb348883, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Immunohistochemical staining of guinea pig skin using anti-CD4 antibody CE9.1 (Clenoliximab) Anti-CD4 staining of formaldehyde fixed paraffin embedded guinea pig skin tissue, at 40x magnification. The human IgG4-chimeric version of CE9.1 (Clenoliximab) (orb348872) was used to stain samples at a concentration of 3 µg/ml.

Western Blot using anti-CD4 antibody CE9.1 (Clenoliximab) Human thyroid tumour samples were resolved on a 15% SDS PAGE gel and blots probed with the human IgG4-chimeric version of CE9.1 (orb348872) at 1.5 µg/ml before detection using an anti-human IgG4 secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for CD4 is 51kDa (arrowhead). Here some non-specific bands can also be seen. orb348872 successfully detected CD4 in human thyroid tissue samples.