Anti-CD37 [MB-1]

• Catalog Number: orb758771
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD37
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MB-1
• Tested applications: FC, ICC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: BALB/c mice were immunized with lymph node cells from a patient with nodular poorly differentiated lymphocytic lymphoma. Immunophenotyping studies of these cells identified them as B cells expressing SIgG/λ, as well as HLA-DR. Leu-12, and B1.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD37
• UniProt ID: P11049
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Tetraspanin-26; Tspan-26; Leukocyte antigen CD37;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-CD37 antibody MB-1. Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (orb256449; isotype control, black line) or the mouse IgG1 version of MB-1 (orb758771, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of Daudi cells using anti-CD6 OX-126. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells stained with the chimeric mouse IgG version of OX-126 (orb758771) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758771, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (orb256449) followed by Alexa Fluor® 488 secondary antibody.