Anti-CD36 [185-1G2 (B467)]

• Catalog Number: orb758920
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD36
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 185-1G2 (B467)
• Tested applications: Blocking, FC, IF, IHC, IP
• Reactivity: Human
• Isotype: IgM
• Immunogen: This antibody was raised by immunizing mice with stimulated human leukocytes.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD36
• UniProt ID: Q8WTV0
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: SRB1; Scavenger receptor class B member 1; CD36 an
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed HepG2 cells with anti-CD36 antibody 185-1G2 (B467). Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 185-1G2 (B467) (orb758921) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758921, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CD36 antibody 185-1G2 (B467) Human peripheral blood monocyte cell lysate (35 µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 185-1G2 (B467) (orb758921) at 0.03 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.