Anti-CD33 [hP67.6 (Gemtuzumab)]

• Catalog Number: orb1152519
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD33
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: hP67.6 (Gemtuzumab)
• Tested applications: FC, IF
• Reactivity: Human
• Isotype: Human IgG1
• Immunogen: Gemtuzumab was generated by huminization of the murine P67.6 clone, raised against CD33-transfected FMY9S5 cells of human origin. CD33 is a transmembrane receptor expressed on predominantly on cells of the myeloid lineage, but it can also be found on some lymphoid cells.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD33
• UniProt ID: P20138
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Siglec-3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Binding curve of anti-CD33 antibody hP76.6 (Gemtuzumab) to human CD33 ELISA Plate coated with human His-tagged CD33 at a concentration of 2 µg/ml. A 3-fold serial dilution from 10000 to 0.1 ng/ml. For detection, a 1:4000 dilution of HRP-labelled anti-human kappa light chain antibody was used.

Flow-cytometry using anti-CD33 antibody hP67.6 (Gemtuzumab) Human leukocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of hP67.6 (Gemtuzumab) (orb411568, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.