Anti-CD32 [IV.3]

• Catalog Number: orb1152472
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD32
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: IV.3
• Tested applications: Blocking, ELISA, IF, IP
• Reactivity: Human
• Isotype: IgG2b
• Immunogen: The original antibody was generated against the FcR of K562 cells.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD32
• UniProt ID: P12318
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CDw32; Fc-gamma RII-a; FcγRIIa; FcγRII; FcRII-a; F
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-CD32 antibody IV.3. Paraformaldehyde fixed U937 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of IV.3 (orb1152473, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of U937 cells with anti-CD32 IV.3. Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of IV.3 (orb1152473) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1152473, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.