Anti-CD31 [BAG-85D10]

• Catalog Number: orb758909
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD31
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: BAG-85D10
• Tested applications: IHC
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: This antibody was raised by immunising mice with human peripheral blood lymphocytes from a patient with high numbers of gamma-delta T cells.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD31
• UniProt ID: P16284
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: PECAM1; Platelet endothelial cell adhesion molecul
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-CD31 antibody BAG-85D10. Paraformaldehyde fixed Jurkat cells were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of BAG-85D10 (orb758909, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed K562 cells with anti-CD31 antibody BAG-85D10. Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of BAG-85D10 (orb758909) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758909, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.