Anti-CD27 [LG.3A10]

• Catalog Number: orb613904
• Category: Antibodies
• Description: Hamster monoclonal antibody to CD27
• Species/Host: Hamster
• Clonality: Monoclonal
• Clone Number: LG.3A10
• Tested applications: FC, IF, IP
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: This antibody was produced by injecting Armenian hamsters intraperitoneally with a mixture of two murine CD27 tag-expressing Armenian hamster fibroblast clones (AR0524 and AR0530) in Freund's adjuvant
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD27
• UniProt ID: P41272
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TNFRS7; CD27L receptor; T-cell activation antigen
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Binding curve of anti-CD27 antibody LG.3A10 (orb613905) to mouse CD27-Fc fusion protein ELISA Plate coated with mouse CD27-Fc fusion protein at a concentration of 5 µg/ml. A 3-fold serial dilution from 10000 to 0.1 ng/ml was performed using orb613905. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody was used.

Immunofluorescence staining of fixed mouse splenocytes with anti-CD27 antibody LG.3A10. Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix™ coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of LG.3A10 (orb613905) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613905, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.