Anti-CD27 antibody(DM57); Rabbit mAb

• Catalog Number: orb757380
• Category: Antibodies
• Description: Anti-CD27 antibody(DM57), Rabbit mAb
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: DM57
• Tested applications: ELISA, FC
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Recombinant Antibody
• Dilution range: ELISA 1/5000-10000|Flow Cyt 1/100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CD27
• UniProt ID: P26842
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD27, TNFRSF7, S152, T14, Tp55
• Note: For research use only
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Expiration Date: 12 months from date of receipt.

Expi 293 cell line transfected with irrelevant protein (A) and human CD27 (B) were surface stained with Rabbit anti-CD27 monoclonal antibody 1 μg/ml (clone: DM57) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Flow cytometry data of serially titrated Rabbit anti-CD27 monoclonal antibody (clone: DM57) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Affinity ranking of different Rabbit anti-CD27 mAb clones by titration of different concentration onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Flow cytometry analysis of antigen binding of rabbit anti-human CD27 mAb (orb757380). (A) orb757380 does not bind to 293T cells that do not express CD27. (B) A clear peak shift of orb757380 was seen compared to the control when incubated with CD27-expressing Raji cells, indicating strong binding of orb757380 to CD27. Antibodies were incubated at 2 μg/mL.