Anti-CD169 [3D6.112 (recombinant version)]

• Catalog Number: orb613783
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD169_Sialoadhesin
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: 3D6.112 (recombinant version)
• Tested applications: IHC, WB
• Reactivity: Mouse
• Isotype: Rat IgG
• Immunogen: Spleen cells from AO rats immunised with thioglycollate-elicted murine peritoneal macrophages (TPM) that had been cutured in mouse serum to induce sheep erythrocyte receptor (SER).
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD169
• UniProt ID: Q62230
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Sialoadhesin; Sheep erythrocyte receptor; SER; Sia
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-CD169 staining of murine macrophages Murine bone marrow-derived macrophages (BMDMs) were stained with anti-CD169 antibody orb613784 (as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor® 647 (AF647) commercially available from a competitor. Compared to the isotype control and secondary-only control, a CD169-positive population is apparent.