Anti-CD163 [2A10 (2A10/11)]

• Catalog Number: orb1152468
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD163
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2A10 (2A10/11)
• Tested applications: ELISA, FC, IF
• Reactivity: Porcine
• Isotype: Mouse IgG
• Immunogen: The original antibody was generated by immunizing BALB/c mice with porcine alveolar macrophages.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD163
• UniProt ID: Q2VL90
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: M130; sCD163; Scavenger receptor cysteine-rich typ
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-CD163 antibody 2A10 (2A10/11). Paraformaldehyde fixed pig peripheral blood monocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of 2A10 (2A10/11) (orb1152468, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of pig peripheral blood monocytes with anti-CD163 2A10 (2A10/11). Immunofluorescence analysis of paraformaldehyde fixed pig peripheral blood monocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 2A10 (2A10/11) (orb1152468) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1152468, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.