Anti-CD123 (talacotuzumab biosimilar) mAb

• Catalog Number: orb689308
• Category: Antibodies
• Description: Anti-CD123 (talacotuzumab biosimilar) mAb
• Species/Host: Human Humanized
• Clonality: Monoclonal
• Tested applications: ELISA, FC
• Reactivity: Human
• Isotype: IgG1
• Dilution range: ELISA 1:5000-10000|Flow Cyt 1:100
• Form/Appearance: Powder
• Conjugation: Unconjugated
• Target: CD123
• UniProt ID: P26951
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD123;IL3R;IL3RA
• Note: For research use only
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Expiration Date: 12 months from date of receipt.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD123, hFc-His tagged protein (orb689364) can bind Anti-CD123 Neutralizing antibody in a linear range of 0.98-26.70 ng/ml. In order to specifically detect orb689308, mouse anti-human Fab-specific antibody was used as detection antibody.

HEK293 cell line transfected with irrelevant protein (B) and human CD123 (A) were surface stained with anti-CD123 neutralizing antibody 1 μg/ml (talacotuzumab) followed by Alexa 488-conjugated anti-human IgG secondary antibody.

Flow cytometry analysis of antigen binding of anti-human CD123 mAb (orb689308). (A) A clear peak shift of orb689308 was seen compared to the control when incubated with CD123-expressing 8226 cells, indicating strong binding of orb689308 to CD123. Antibodies were incubated at 2 μg/mL.