Anti-CD11c [N418]

• Catalog Number: orb613842
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD11c
• Species/Host: Hamster
• Clonality: Monoclonal
• Clone Number: N418
• Tested applications: FACS, FC, IF, In vivo
• Reactivity: Human, Mouse
• Isotype: Hamster IgG
• Immunogen: Hamster spleen cells that had been primed to mouse spleen dendritic cells
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD11c
• UniProt ID: P20702
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Integrin alpha-X
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed U937 cells with anti-CD11c antibody N418. Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of N418 (orb613842) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613842, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.