Anti-CD11c Antibody

• Catalog Number: orb665908
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CD11c
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, WB
• Reactivity: Mouse, Rat
• Immunogen: Recombinant protein of human CD11c. The exact sequence is proprietary.
• Dilution range: WB: 1-500-2000, IHC-P: 1-50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ITGAX
• Entrez: 3687, 16411
• UniProt ID: P20702, Q9QXH4
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-CD11C antibody, anti-Integrin alpha-X antibod
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CD11c expression in THP1 (A), mouse spleen (B) whole cell lysates. (Predicted band size: 127 kD; Observed band size: 150 kD)

Immunohistochemical analysis of CD11c staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of CD11c staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).