Anti-CD11b [5C6 (recombinant version)]

• Catalog Number: orb613781
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD11b
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: 5C6 (recombinant version)
• Tested applications: IF, IHC-Fr
• Reactivity: Human, Mouse
• Isotype: Rat IgG
• Immunogen: Fusion of spleen cells from AO rats immunised with Thioglycollate-elicted muine peritoneal macrophages (TPM) and the Y3 rat myeloma line.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD11b
• UniProt ID: P11215
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: integrin alpha M; CD11 antigen-like family member
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-CD11b staining of murine neutrophils. Ex vivo murine (C57BL6/J) neutrophils from zymosan-elicited peritoneal exudate were stained with anti-CD11b antibody and analysed by flow-cytometry. Forward- and side-scatter was used to gate on neutrophil population (data not shown) and fluorescence intensity analysed. Left panel shows the signal obtained from cells pre-treated with Fc-blocking reagent and subsequently stained with anti-CD11b (hybridoma-derived antibody obtained from competitor, clone M1/70) directly conjugated to PerCP. Comparison to unstained cells shows that that cells analysed are predominantly CD11b-positive. Right panel shows staining first with anti-CD11b (orb613782, clone 5C6 (recombinant version)) or isotype control (orb256396) followed by staining with fluorescently conjugated secondary antibody (goat anti-rat). As a further control, cells were also only stained with secondary antibody. Comparison of the staining pattern obtained using orb613782 compared to isotype- and secondary-only-control shows that the analysed cells are predominantly Cd11b-positive, agreeing with findings obtained using hybridoma-derived antibody.