Anti-CD11a [hu1124 (Efalizumab)]

• Catalog Number: orb348923
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CD11a
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: hu1124 (Efalizumab)
• Tested applications: Blocking, FC
• Reactivity: Human
• Isotype: Human IgG
• Immunogen: Efalizumab binds to CD11a, a subunit of leukocyte function antigen-1 (LFA-1), which is expressed on all leukocytes.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD11a
• UniProt ID: P20701
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: LFA-1; integrin alpha L
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Bauer, R. J. et al. Population pharmacokinetics and pharmacodynamics of the anti-CD11a antibody hu1124 in human subjects with psoriasis J Pharmacokinet Biopharm, 27, 397-420 (1999)

PubMed: 10826130

Reactivity

Human

Flow-cytometry using the anti-CD11a research biosimilar antibody Efalizumab (hu1124) Human lymphocytes were stained with an isotype control (orb256390, panel A) or the rabbit-chimeric version of Efalizumab (orb348923, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Immunohistochemical staining of rat spleen tissue using anti-CD11a antibody hu1124 (Efalizumab). Anti-CD11a staining of formaldehyde fixed paraffin embedded rat spleen tissue, at 40x magnification. The human IgG1-chimeric version of hu1124 (orb348868) was used to stain samples at a concentration of 3 µg/ml.