Anti-CD115/M-CSFR [AFS98]

• Catalog Number: orb758901
• Category: Antibodies
• Description: Rat monoclonal antibody to CD115
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: AFS98
• Tested applications: Blocking, FC, IHC, WB
• Reactivity: Mouse
• Isotype: IgG2a
• Immunogen: This antibody was raised against CD115.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CD115/M-CSFR
• UniProt ID: P09581
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CSF-1R; Macrophage colony-stimulating factor 1 rec
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-CD115/M-CSFR antibody AFS98. Paraformaldehyde fixed mouse splenocytes were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of AFS98 (orb758900, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed RAW264.7 cells with anti-CD115/M-CSFR antibody AFS98. Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of AFS98 (orb758900) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758900, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.