Anti-CD115 Antibody

• Catalog Number: orb393256
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CSF1R.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat, Zebrafish
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD115. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500:1000, IHC-P: 1:100:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CSF1R
• Entrez: 12978, 1436
• UniProt ID: P07333, Q00495, P09581
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: FMS; Macrophage colony-stimulating factor 1 recept
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Meng, Huiling et al. Knockout of zebrafish colony-stimulating factor 1 receptor by CRISPR/Cas9 affects metabolism and locomotion capacity Biochem Biophys Res Commun, 551, 93-99 (2021)

PubMed: 33725575

Western blot analysis of CD115 expression in A549 (A) whole cell lysates. (Predicted band size: 107 kD; Observed band size: 175 kD)

Immunohistochemical analysis of CD115 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.