Anti-Cardiac Troponin I [scFv 180]

• Catalog Number: orb613846
• Category: Antibodies
• Description: Mouse monoclonal antibody to Cardiac Troponin I
• Species/Host: Gallus
• Clonality: Monoclonal
• Clone Number: scFv 180
• Tested applications: WB
• Reactivity: Human
• Isotype: Gallus IgG1
• Immunogen: Cardiac Troponin I peptide (KISASRKLQLKT).
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: Cardiac Troponin I
• UniProt ID: P19429
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TNNI3; cTnI; cTn-I
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed HeLa cells with anti-Cardiac Troponin I antibody scFv 180. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of scFv 180 (orb613846) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613846, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.

Western Blot using anti-Cardiac Troponin I antibody scFv 180 Human heart lysate (35 µg protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of scFv 180 (orb613846) at 0.001 µg/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for Cardiac Troponin I is 24.0 kDa. orb613846 successfully detected Cardiac Troponin I in human heart lysate.