Anti-beta-Tubulin Purified

• Catalog Number: orb44542
• Category: Antibodies
• Description: Mouse Monoclonal to beta tubulin.
• Clonality: Monoclonal
• Clone Number: TU-13
• Tested applications: ICC, IHC-Fr, WB
• Reactivity: Human, Mouse, Plant, Porcine
• Isotype: Mouse IgM
• Immunogen: beta-tubulin from porcine brain
• Concentration: 1 mg/ml
• Dilution range: Western blotting: Recommended dilution: 1-2 μg/ml.
• Purity: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated
• Target: beta-Tubulin
• Entrez: 81027
• UniProt ID: Q9H4B7
• RRID: AB_10995577
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Alternative names: Anti-beta-tubulin antibody, anti-TUBB antibody
• Note: For research use only
• Application notes: Western blotting: Recommended dilution: 1-2 μg/ml.
• Expiration Date: 12 months from date of receipt.

Anti-beta-Tubulin Purified (TU-13) works in WB application under reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of HeLa, HEK 293, ESS-1 and Jurkat cell lines mixed and heated (100°C, 5 min) with reducing (2-mercaptoethanol) or non-reducing SDS-loading buffer. Samples were resolved using 12% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed simultaneously with mouse IgM monoclonal antibody TU-13 (1 µg/ml) and mouse IgG1 anti-GAPDH monoclonal antibody FF26A (1 µg/ml) used as the loading control. Subclass-specific secondary antibodies IRDye 680RD Goat-anti-Mouse IgM (red) and IRDye 800CW Goat-anti-Mouse IgG (green) were used for multiplex fluorescent Western blot detection. Beta-tubulin was detected at ~50 kDa in all tested cell lines.