Anti-beta-Tubulin Purified

• Catalog Number: orb44541
• Category: Antibodies
• Description: Mouse Monoclonal to beta tubulin.
• Clonality: Monoclonal
• Clone Number: TU-12
• Tested applications: FC, ICC, WB
• Reactivity: Human, Mouse, Plant, Porcine, Rat
• Isotype: Mouse IgM
• Immunogen: Porcine brain microtubule protein MTP-1.
• Concentration: 1 mg/ml
• Dilution range: Immunocytochemistry: Recommended dilution: 2-5 μg/ml, fixed and permeabilized cells. Western blotting: Recommended dilution: 1-2 μg/ml, reducing conditions. Flow cytometry: Recommended dilution: 4-10 μg/ml. Intracellular staining.
• Purity: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated
• Target: beta-Tubulin
• Entrez: 81027
• UniProt ID: Q9H4B7
• RRID: AB_10994817
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Alternative names: Anti-beta-tubulin antibody
• Note: For research use only
• Application notes: Immunocytochemistry: Recommended dilution: fixed and permeabilized cells.
• Expiration Date: 12 months from date of receipt.

Separation of MCF-7 cells stained using anti-beta Tubulin (TU-12) purified antibody (concentration in sample 8 µg/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).