Anti-Bet v 1 [M0418]

• Catalog Number: orb613898
• Category: Antibodies
• Description: Human monoclonal antibody to Bet v 1
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: M0418
• Tested applications: ELISA
• Reactivity: Plant
• Isotype: IgE
• Immunogen: Bet v 1-A, a major allergen of the European white birch.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: Bet v 1
• UniProt ID: P15494
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Major Allergen from Birch Pollen; BETVIA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Binding curve of anti-Bet v 1 antibody M0418 (orb613899) to Bet v 1 ELISA Plate coated with Bet v 1-A at a concentration of 2 µg/ml. A 3-fold serial dilution from 10000 to 0.1 ng/ml was performed using orb613899. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody was used. Absorbance at 450nm does not reach a plateau but falls slightly before increasing once more, as also observed for orb613897. This is suggestive of two binding sites on Bet v 1-A for which orb613899 has differing affinities.