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Anti-ATP1A1 (Phospho-S23) Antibody

Catalog Number: orb665511

DispatchUsually dispatched within 5-10 working days
$ 160.00
Catalog Numberorb665511
CategoryAntibodies
DescriptionRabbit polyclonal antibody to ATP1A1 (Phospho-S23)
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, WB
ReactivityHuman, Mouse, Rat
ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S23 of human ATP1A1 protein. The exact sequence is proprietary.
Dilution rangeWB: 1-500-1000, IF: 1-100-500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetATP1A1
Entrez24211
UniProt IDP06685
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesAnti-ATP1A1 antibody, anti-Sodium/potassium-transp
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-ATP1A1 (Phospho-S23) Antibody

Western blot analysis of ATP1A1 (Phospho-S23) expression in HEK293T (A), MCF7 (B), DLD (C), SGC7901 (D) whole cell lysates. (Predicted band size: 112 kD; Observed band size: 100 kD)

Anti-ATP1A1 (Phospho-S23) Antibody

Immunofluorescent analysis of ATP1A1 (Phospho-S23) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.