Anti-ATP1A1 (Phospho-S16) Antibody

• Catalog Number: orb304689
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ATP1A1 (phospho-S16)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Porcine, Rabbit, Rat, Sheep
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S16 of human ATP1A1 protein. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ATP1A1
• Entrez: 24211, 11928, 476
• UniProt ID: Q8VDN2, P06685, P05023
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti Sodium/potassium-transporting ATPase subunit
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ATP1A1 (Phospho-S16) expression in HEK293T (A), HCT116 (B), Panc1 (C), PC12 (D), CT26 (E) whole cell lysates. (Predicted band size: 112 kD; Observed band size: 112 kD)

Immunohistochemical analysis of ATP1A1 (Phospho-S16) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.