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Anti-ATP1A1 Antibody

Catalog Number: orb235028

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb235028
CategoryAntibodies
DescriptionRabbit polyclonal antibody to ATP1A1
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Porcine, Rabbit, Rat, Sheep
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ATP1A1. The exact sequence is proprietary.
Dilution rangeWB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetATP1A1
Entrez11928, 476, 24211
UniProt IDP05023, P06685, Q8VDN2
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti Sodium/potassium-transporting ATPase subunit
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-ATP1A1 Antibody

Western blot analysis of ATP1A1 expression in mouse kidney (A), mouse muscle (B) whole cell lysates. (Predicted band size: 112 kD; Observed band size: 100 kD)

Anti-ATP1A1 Antibody

Immunohistochemical analysis of ATP1A1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-ATP1A1 Antibody

Immunofluorescent analysis of ATP1A1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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    ELISA,  IF,  WB

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