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Anti-Aquaporin 2 (Phospho-S256) Antibody

Catalog Number: orb1289875

DispatchUsually dispatched within 5-10 working days
$ 190.00
Catalog Numberorb1289875
CategoryAntibodies
DescriptionRabbit Polyclonal antibody to Aquaporin 2
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIH, WB
ReactivityHuman, Mouse, Porcine, Rat, Sheep
ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S256 of human Aquaporin 2 protein. The exact sequence is proprietary.
Dilution rangeWB: 1:500-1:1000, IH: 1:50-1:200
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetAQP2
Entrez25386, 359, 11827
UniProt IDP56402, P41181, P34080
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesAquaporin-2; AQP-2; ADH water channel; Aquaporin-C
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-Aquaporin 2 (Phospho-S256) Antibody

Western blot analysis of Aquaporin 2 (Phospho-S256) expression in mouse kidney (A), rat kidney (B), mouse lung (C) whole cell lysates. (Predicted band size: 28 kD; Observed band size: 37; 28 kD)

Anti-Aquaporin 2 (Phospho-S256) Antibody

Immunohistochemical analysis of Aquaporin 2 (Phospho-S256) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.