Anti-ANP32E Antibody

• Catalog Number: orb1879780
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ANP32E
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human ANP32E. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/10 - 1/50)
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Entrez: 66471, 81611
• UniProt ID: Q9BTT0, P97822
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Alternative names: Acidic leucine-rich nuclear phosphoprotein 32 fami
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ANP32E expression in SHSY5Y (A), U937 (B), mouse brain (C) whole cell lysates. (Predicted band size: 30 kD; Observed band size: 26 kD)

Immunofluorescent analysis of Anti-ANP32E staining in SHSY5Y cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).