Anti-Angiotensinogen/AGT Antibody

• Catalog Number: orb1743974
• Category: Antibodies
• Description: Anti-Angiotensinogen/AGT Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Human
• Immunogen: E.coli-derived human Angiotensinogen/AGT recombinant protein (Position: D273-R458).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 53 kDa
• UniProt ID: P01019
• Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Note: For research use only
• Application notes: Western blot, 0.1-0.25 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human Direct ELISA, 0.1-0.5 μg/ml, Human. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HepG2 cells using anti-Angiotensinogen/AGT antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis using anti-Angiotensinogen/AGT antibody.Lane 1:human hepatocellular carcinoma tumor tissue (HCCT) lysates;2:human hepatocellular carcinoma paracancerous tissue (HCCP);3:human HepG2 cell;4:human HUH-7 cell.

IHC analysis of Angiotensinogen/AGT using anti-Angiotensinogen/AGT antibody. Angiotensinogen/AGT was detected in a paraffin-embedded section of human lung adenocarcinoma tissue.

IHC analysis of Angiotensinogen/AGT using anti-Angiotensinogen/AGT antibody. Angiotensinogen/AGT was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue.