Anti-AMPK beta 2 Antibody

• Catalog Number: orb381985
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PRKAB1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human AMPK beta 2
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PRKAB2
• Entrez: 64562, 5565, 108097
• UniProt ID: O43741, Q9QZH4, Q6PAM0
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-1300015D22Rik antibody, anti-5''-AMP-activate
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Brian Z. Wang, Jane J. Yang et al. AMPK Signaling Regulates the Age-Related Decline of Hippocampal Neurogenesis Aging and disease, 0

PubMed: 31595203

Western blot analysis of AMPK beta 2 expression in MCF7 (A), mouse heart (B), rat heart (C) whole cell lysates. (Predicted band size: 21; 30 kD; Observed band size: 33 kD)

Immunofluorescent analysis of AMPK beta 2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.