Anti-alpha/beta-Tubulin dimer Purified

• Catalog Number: orb44536
• Category: Antibodies
• Description: Mouse Monoclonal to alpha/beta Tubulin dimer.
• Clonality: Monoclonal
• Clone Number: TU-08
• Tested applications: ICC, WB
• Reactivity: Human, Mouse, Porcine
• Isotype: Mouse IgM
• Immunogen: Microtubule proteins from porcine brain.
• Concentration: 1 mg/ml
• Purity: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated
• Target: alpha/beta-Tubulin dimer
• RRID: AB_10993516
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Alternative names: Anti-alpha antibody, anti-beta-tubulin dimer antib
• Note: For research use only
• Application notes: Western blotting: Recommended dilution: 5 μg/ml; positive control: porcine brain lysate, reducing conditions.
• Expiration Date: 12 months from date of receipt.

Western blotting analysis of human alpha/beta-tubulin using mouse monoclonal antibody TU-08 on lysates of various cell lines and porcine brain under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-alpha/beta-tubulin monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. Alpha/beta-tubulin was detected at approximately 54 kDa.